Genome-wide methylation study in intracranial germ cell tumors

2014 Fiscal Year Final Research Report

• Project/Area Number: 25830093
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: National Cancer Center Japan
• Principal Investigator: SHINTARO Fukushima 独立行政法人国立がん研究センター, 研究所, リサーチレジデント (30529459)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 頭蓋内胚細胞腫 / ジャーミノーマ / ゲノムワイドな低メチル化 / 始原生殖細胞由来

Outline of Final Research Achievements

To elucidate the pathogenesis of intracranial germ cell tumors (iGCTs), we are going to execute a comprehensive genomic analysis by multi-institusional study. We used genomic DNA extracted from each frozen samples of 25 germinomas, 36 non-germinomatous GCTs (NGGCTs), and 6 normal tissues. A genome-wide methylation status was analyzed using HumanMethylation450 BeadChip (Illumina@). Germinomas were characterized by a global DNA hypomethylation in comparison to NGGCTs and normal tissues, which suggested germinomas originate from primordial germ cell showing a global hypomethylation during mammalian embryogenesis.

Free Research Field

脳腫瘍のゲノム解析