Comprehensive analysis of MHC-class I-restricted cancer antigen peptides

2014 Fiscal Year Final Research Report

• Project/Area Number: 25830108
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: National Cancer Center Japan
• Principal Investigator: NARUMI Kenta 独立行政法人国立がん研究センター, その他部局等, 研究員 (10534258)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ペプチドワクチン / がん抗原 / 循環血中腫瘍細胞

Outline of Final Research Achievements

Here we reported an acid-stripping-mediated comprehensive analysis method of MHC class I-restricted cancer antigen peptides. We added five HLA-A24-restricted Glypican3-related peptides peptides to the HLA-A24-expressing cells, following acid-stripping and ultrafiltration. The filtrates were analyzed by 2DICAL. It was revealed that only the peptide which had the strongest binding affinity against the HLA-A24 molecules was detected in the filtrates. This finding indicated that acid-stripping and 2DICAL analysis was a useful method to detect HLA-A24-binding peptides. This method may be applicable to the comprehensive screening of cancer antigen using circulating tumor cells.

Free Research Field

腫瘍免疫