2015 Fiscal Year Final Research Report
Cell cycle- and differentiation-dependent transcriptional regulation by nuclear envelope
| Project/Area Number |
25840008
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 核膜 / ラミンＢ受容体 / 転写制御 / ヘテロクロマチン / 細胞外マトリクス |
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| Outline of Final Research Achievements |
In this study, I attempted to reveal how the nuclear envelope regulates transcription thorough analyzing the gene regulation mechanism by one of the nuclear membrane proteins, lamin B receptor (LBR), at genome-wide level. I investigated the relationship between the LBR-binding and the transcriptional level of the gene across the whole genome before and after differentiation of HL60 cells by retinoic acid treatment, and found that LBR regulated extracellular matrix-related genes and its pathway. Those genes were also varied in LBR-knocked down HeLa cells. Taken together, those results suggested that the nuclear envelope plays a role to create an extracelluar environment in response to a biological signal such as differentiation.
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| Free Research Field |
生化学、分子生物学、細胞生物学
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