2015 Fiscal Year Final Research Report
Regulatory mechanism controlling a novel nuclease activity of Mre11 complex on structured DNA
| Project/Area Number |
25840009
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
FURUKOHRI Asako 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (90546293)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | DNA修復 / Mre11 complex / DNA二本鎖切断 |
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| Outline of Final Research Achievements |
The Mre11/Rad50 complex has important roles in various genome maintenance pathways. In this research project, we analyzed a novel type of nuclease activity found for Escherichia coli Mre11/Rad50 complex, SbcCD. Unexpectedly, nuclease activities of SbcCD were not significantly affected by a secondary structure on substrate DNA, but we found that the activity of SbcCD is modulated by the presence of DNA end. SbcCD specifically cuts off the top of a cruciform DNA or the end of dsDNA by making incisions on both strands. The finding of this a novel, DNA-end dependent nuclease activity will provide new insights in the future research and may help us understand the role of the Mre11/Rad50 complex in the human cell.
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| Free Research Field |
分子生物学
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