2014 Fiscal Year Final Research Report
Development of the new method for dynamics analysis in large molecular protein-protein complex using nuclear magnetic resonance
| Project/Area Number |
25840021
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
MIYANOIRI Yohei 名古屋大学, 理学(系)研究科(研究院), 特任助教 (80547521)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | NMR / 蛋白質動態 |
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| Outline of Final Research Achievements |
To elucidate the dynamic properties of GroEL-ES protein complexs, We have developed new relaxation optimized Stereo-Array Isotope labelling (SAIL) method. Using new relaxation optimized SAIL amino acids, we could succeed in observing well-separeted aromatic, aliphatic and methyl CH signals for GroEL-ES complex. And we could detect the changes in structural flactuations in several amino acid s in GroEL and GroES upon forming GroEL-ES complex. These dynamics may play an important role for cotrolling a biological function of GroEL-ES complex.
Our new SAIL NMR method will be useful for structural dynamics study in many macromolecular protein complexes.
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| Free Research Field |
構造生物学
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