Improvement of multi-photon excitation microscopy for controlling single cell cAMP level in deep tissue

2014 Fiscal Year Final Research Report

• Project/Area Number: 25840044
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Hokkaido University
• Principal Investigator: OTOMO Kohei 北海道大学, 電子科学研究所, 特任助教 (40547204)
• Co-Investigator(Renkei-kenkyūsha): NEMOTO Tomomi 北海道大学, 電子科学研究所, 教授 (50291084) ; ISEKI Mineo 東邦大学, 薬学部, 准教授 (60414009) ; WATANABE Masakatsu 光産業創成大学院大学, 特任教授 (40144226)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 多光子顕微鏡 / 超解像顕微鏡 / 光遺伝学

Outline of Final Research Achievements

The research goal was to develop new multi-photon excitation fluorescence microscopy systems which are beneficial for optogenetics studies. Specifically, we aimed to develop a microscopy system that enables light activation control of cyclic adenosine monophosphate (cAMP) level in the single cell locating at the deep region of a live specimen, and visualization of the changes of cAMP level at the high spatiotemporal resolution. By utilizing a multipoint laser scanning method, development of a microscopy system that enables the high temporal resolution multi-photon excitation fluorescence imaging and the locally multi-photon activation was achieved. Furthermore, by utilizing a stimulated emission depletion process, a new two-photon excitation super-resolution microscopy technique was developed. At present, we approach to locally cAMP control in a live specimen by use of developed microscopy systems.

Free Research Field

生物物理学