semi-in vivo analysis on structural dynamics of ribosome complex by mass spectrometry

2015 Fiscal Year Final Research Report

• Project/Area Number: 25840052
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Suntory Foundation for Life Sciences (2014-2015); Keio University (2013)
• Principal Investigator: Yamamoto Tatsuya 公益財団法人サントリー生命科学財団, 統合生体分子機能研究部, 研究員 (70437573)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: リボソーム / 構造機能相関

Outline of Final Research Achievements

About macro protein complexes, we developed a new method for directly-extracted ribosomes from unicellular organism (E.coli) and higher animal cells (HEK293: human cultured cell) by mass spectrometry and H/D exchange, to progress the function analysis in the cell through the structural analysis. We succeeded in development of a method, which detects structural changes of many ribosomal proteins from sonicated cell extracts under 1mg cells, by rough purification and optimization of MALDI matrix. This developed method suggested a possibility for the directly detection of structural change from individual target without the gene cloning and over expressions.

Free Research Field

生物物理学