2014 Fiscal Year Final Research Report
Elucidation of operation mechanism of rotary molecular motors by comparison with the mechanisms of F1 and V1-ATPases.
| Project/Area Number |
25840053
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Chuo University |
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Principal Investigator |
UENO Hiroshi 中央大学, 理工学部, 助教 (10546592)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 1分子計測 / 分子モーター |
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| Outline of Final Research Achievements |
We have succeeded in analyzing the ATP-driven rotation of Enterococcus hirae V1-ATPase at single-molecule level for the first time. The detailed analysis of the rotation and the comparison with V1-ATPase from another species revealed the common rotary mechanism of V1-ATPase. Furthermore, we determined the rotary angle at which the elementary reactions of ATP hydrolysis occur. These results lead to a deeper understanding of operation mechanism of rotary molecular motors.
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| Free Research Field |
生物物理
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