Identification of factors required for plant fertilization by using a new phenomenon, fertilization independent ovule enlargement.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25840106
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: KASAHARA Ryushiro 名古屋大学, トランスフォーマティブ生命分子研究所, 招へい教員 (40467270)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 植物の受精 / 受精不全変異体 / バニリン染色 / 受精非依存的種子肥大 / 雌性配偶体 / 雄性配偶体

Outline of Final Research Achievements

Very few genes required for plant fertilization are known and to understand the molecular mechanism of it, I need to obtain the factors required for plant fertilization. To obtain the factors, I started a screening by using a very effective 'vanillin staining method' to distinguish fertilized ovules from unfertilized ovules. I have already screened 13698 plants and identified 382 mutants. Of these mutants, I observed and checked 9 mutants and identified 7 female gametophytic mutants and 2 male gametophytic mutants. It is clear that the 'vanillin staining method' is very efficient to identify mutants defective in plant fertilization.

Free Research Field

植物分子生物学