2016 Fiscal Year Final Research Report
Genetic mechanisms of speciation in two closely related bitterling fishes
| Project/Area Number |
25840145
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | Osaka Medical College |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
|
| Keywords | 魚類 / タナゴ亜科 / 進化 / 種分化 / 生殖隔離 / RNA-Seq / ゲノム解析 |
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| Outline of Final Research Achievements |
Reproductive isolation of two closely related bitterlings Tanakia lanceolata and T. limbata, small cyprinid fishes, is known to be incomplete and their hybrids are frequently observed in the wild. To understand the speciation mechanisms of these fishes, phylogenetic and evolutionary analyses were carried out. Phylogenetic analysis by RNA-Seq data suggested that many genes were affected by past hybridization events in these species. Hybrid discrimination analysis using divergent genetic markers showed that frequency of hybridization is highly different among regional populations. F2 hybrids of T. lanceolata and T. limbata have been generated by artificial fertilization. Construction of a linkage map in these F2 hybrids is now in progress.
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| Free Research Field |
進化生物学
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