Construction of novel hybrid Agrobacterium strains useful for plant transformation.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25850003
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: Hiroshima University
• Principal Investigator: YAMAMOTO Shinji 広島大学, 理学(系)研究科(研究院), 助教 (50607348)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 形質転換 / アグロバクテリア / Tiプラスミド / Riプラスミド

Outline of Final Research Achievements

In this study, I examined capability of untouched rhizobium strains for a plant transformation technology. Firstly, I established a pathogenic (Ti/Ri) plasmids exchange method, which consists of curing of resident plasmid in recipient cell, addition of transfer origin to the Ti/Ri plasmid in donor cell and conjugation between the donor and the recipient cells. The method was applied to 12 Rhizobium strains for construction of hybrid strains harbor Ti/Ri plasmids from other strains instead of an original one. Total 61 strains including wild type and Ti/Ri-less strains were checked their transformation characteristics, such as an expression of virulence (vir) genes induced by phenolics. As a result, candidate strains useful for transformation, such as the strain strongly expresses vir genes by cocultivation with rice cultured cells, were obtained. The Ti/Ri plasmids exchange method enable us to evaluate the potential characteristics useful for transformation in diverse rhizobium strains.

Free Research Field

微生物学