Perturbation of host developmental pathways by virus infection in plant

2014 Fiscal Year Final Research Report

• Project/Area Number: 25850030
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: ATSUMI Go 公益財団法人岩手生物工学研究センター, 園芸資源研究部, 研究員 (90547217)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 植物ウイルス / 木部 / 木質細胞 / 形態形成 / 二次細胞壁 / 病徴

Outline of Final Research Achievements

Kobu-sho is a syndrome that causes tumorous or hyperplastic disorders on stems, nodes and roots of gentians. Previously, I identified GK32 gene fragment inducing Kobu-sho-like symptom from Gentian Kobu-sho-associated virus genome. In this study, I aimed to elucidate the mechanism that GK32 protein induced Kobu-sho disease. Gene expression analysis suggested that expression of GK32 gene fragment activated the signaling pathway regulating xylem development. I showed that GK32 protein tagged with yellow fluorescent protein formed many speckles or plate-like structures in the nucleus. GK32 co-localized with Nucleolin (protein localized in nucleolus) and several mRNA splicing factors. I isolated GK32-interacting proteins including many ribosomal proteins using yeast two-hybrid screening.

Free Research Field

植物ウイルス学