Reverse genetics analysis of the multiplication mechanism of Rice strip virus

2014 Fiscal Year Final Research Report

• Project/Area Number: 25850032
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: ISHIBASHI Kazuhiro 独立行政法人農業生物資源研究所, 植物・微生物間相互作用研究ユニット, 任期付研究員 (20611742)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: マイナス鎖RNAウイルス / 逆遺伝学 / イネ縞葉枯ウイルス / トマト黄化えそウイルス / 出芽酵母

Outline of Final Research Achievements

Becaues of the lack of reverse genetics systems, infection processes of negative-strand RNA viruses of plants are largely unknown. This project was aimed to establish the reverse genetics system of rice stripe virus (RSV) and tomato spotted wilt virus (TSWV) in the family Bunyaviridae. First, I tried to reconstitute the RSV ribonucleoprotein complex in vitro using purified RNA polymerase and nucleocapsid, but the trial was unsuccessful. Then, I expressed RNA polymerase, nucleocapsid, and S RNA of TSWV in Saccharomyces cerevisiae, and found that replication of TSWV S RNA occured. This suggests that TSWV RNP was formed in yeast.

Free Research Field

植物ウイルス学