2015 Fiscal Year Final Research Report
Establishment of a simple identification system for Japanese jumping plant lice through DNA barcode
Project/Area Number |
25850035
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Plant protection science
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Research Institution | National Agriculture and Food Research Organization |
Principal Investigator |
INOUE Hiromitsu 国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門ブドウ・カキ研究領域, 主任研究員 (80414663)
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Research Collaborator |
MIYATAKE Yorio
MATSUMOTO Koichi
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Keywords | DNAバーコーディング / キジラミ / 同定・識別 / 隠蔽種 / ミトコンドリアCOI領域 / 分子分類 |
Outline of Final Research Achievements |
With the aim of establishing a simple identifying system for the Japanese species of jumping plant lice (Hemiptera: Psylloidea) through DNA barcode, 714 bp mitochondrial cytochrome oxidase subunit I (COI) sequences were obtained from 895 individuals of 123 Japanese psylloid species of 30 genera, 6 families. Among the most species analyzed in this study, the mean and maximum of intraspecific COI differences (p-distance) were 0.1% and 1.0%, respectively. In contrast, the COI differences between congeneric species were 3.2-29.0%. It was shown that psylloid species can be distinguished through the use of COI DNA barcode, except in cases of some of the morphological species.
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Free Research Field |
農学
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