Regulation of spore dormancy and germination in a sporangium-forming rare actinomycete Actinoplanes missouriensis

2014 Fiscal Year Final Research Report

• Project/Area Number: 25850046
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: TEZUKA Takeaki 東京大学, 農学生命科学研究科, 助教 (80646414)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 休眠 / 覚醒 / 発芽 / 希少放線菌 / 細胞応答 / 二成分制御系 / 胞子嚢

Outline of Final Research Achievements

HhkA and TcrA are putative hybrid sensor histidine kinase and response regulator of the two-component regulatory system, respectively, and are essential for spore dormancy in sporangia formed by a rare actinomycete Actinoplanes missouriensis. Using the hhkA- and tcrA-disrupted strains, their transcriptomes were compared with the wild-type strain by RNA-seq analysis, revealing that both HhkA and TcrA are involved in the transcription of over two hundred genes. Besides, electrophoretic mobility shift assay showed that TcrA directly binds to the upstream regions of the flagellar genes.

Free Research Field

応用微生物学