Functional analyses of the novel hemoproteins for biomineralizing magnetic particles in magnetotactic bacteria

2015 Fiscal Year Final Research Report

• Project/Area Number: 25850051
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Kanazawa University
• Principal Investigator: TAOKA Azuma 金沢大学, 自然システム学系, 助教 (20401888)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 細菌 / 磁性 / 磁鉄鉱 / ヘム蛋白質 / シトクロム / バイオミネラリゼーション

Outline of Final Research Achievements

Magnetotactic bacteria have unique c-type cytochromes for biomineralizing magnetite crystals. We characterized and analyzed one of the magnetotactic bacteria specific cytochrome c MamP using Magnetospirillum magneticum AMB-1. MamP was localized in cytoplasmic membrane, and was specifically expressed during the exponential growth phase. According to MamP overproduction experiments, we found that MamP is crucial to the normal growth of magnetite crystals during the exponential growth phase. Moreover, we showed that MamP can be involved in electron transfer to denitrification pathway, and proposed the new model for the electron transfer pathway during the biomineralization of magnetite.

Free Research Field

分子微生物学