2014 Fiscal Year Final Research Report
Cellular uptake of novel cell-penetrating peptide "polyhistidine peptide (H16)"
| Project/Area Number |
25850082
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | ペプチド / 細胞膜透過ペプチド / ヒスチジン / ゴルジ体 / リソソーム / 腫瘍 / 薬物輸送システム / DDS |
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| Outline of Final Research Achievements |
Cell-penetrating peptides (CPPs) are arginine/lysine-rich sequences, and they are effectively internalized into cells. On the other hand, in the present study, we found that polyhistidine peptide (H16: HHHHHHHHHHHHHHHH-NH2) was efficiently internalized into the HT1080 human fibrosarcoma cells. Cellular uptake of the H16 peptide was mainly due to micropinocytosis. Although most of the H16 peptide localized in the lysosome and Golgi apparatus, a proportion of the H16 peptide escaped from the macropinosome to the cytoplasm. In a protein transduction study, green fluorescence protein fused to the H16 peptide (GFP-H16) was also internalized into cells. Furthermore, in vivo study showed that the H16 peptide accumulated immediately in tumor tissue and showed long residence-time in the tumor (HT1080 human fibrosarcoma)-bearing mice. The findings suggest that the H16 peptide is novel CPP and represents a promising drug delivery carrier candidate in medical and biotechnological fields.
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| Free Research Field |
ペプチド化学、細胞生物学
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