2014 Fiscal Year Final Research Report
Online evaluation of superoxide radical scavenging activity of vegetables fat-soluble components as studied by HPLC-ESR spin-trapping measurements
| Project/Area Number |
25850089
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Food science
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
YAMAGUCHI Tomoko 京都工芸繊維大学, ベンチャーラボラトリー, 研究員 (90644914)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 流通系ESR / HPLC-ESR / 抗酸化活性 / スーパーオキシドアニオンラジカル / コーヒー酸換算値 / FI-ESR / 青果物 / ガウス線形シミュレーション |
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| Outline of Final Research Achievements |
Recently, we have developed flow-injection-ESR and HPLC-ESR systems special for quantitative spin-trapping ESR detection of the superoxide radical by using DMPO, as a spin-trapping reagent. The flow-ESR techniques were employed to explore potent superoxide radical scavengers involved in foods. In the present study, HPLC-ESR measurements were made for water-soluble components of foods. The determination of antioxidant activity was calculated using ESR line simulation.
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| Free Research Field |
分析化学
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