2014 Fiscal Year Final Research Report
Studies on injury, viable but non-culturable state, and recovery of Campylobacter jejuni
| Project/Area Number |
25850093
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Food science
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| Research Institution | Fukuoka Women's University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | Campylobacter jejuni / heat injury / recovery |
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| Outline of Final Research Achievements |
Heat injury and recovery from heat injury of Campylobacter jejuni was investigated. Heat injured C. jejuni cells were reproducibly generated by heating cell suspension (in PBS) of C. jejuni at 50°C for 5 minutes. Healthy cells retained culturability both on mCCDA and nutrient agar supplemented with 0.3% NaCl (NA). On the other hand, heat injured cells formed colonies only on mCCDA. Heat injured cells recovered cultivability on NA supplemented with 0.25 g/L sodium pyruvate. The results suggested that glycolysis of C. jejuni had been damaged by non-lethal heating. And the addition of sodium pyruvate would be important for the reliable detection of C. jejuni from foods and environments.
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| Free Research Field |
食品微生物学
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