2015 Fiscal Year Final Research Report
Study on improvement of bovine embryonic development under heat stress by regulation of lysosomal enzyme activities
| Project/Area Number |
25850186
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal production science
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| Research Institution | Saga University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 体外受精 / 暑熱ストレス / リソソーム / ウシ受精卵 |
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| Outline of Final Research Achievements |
In this study, in order to obtain the fundamental information for elucidating the relationship of intracellular digestion and preimplantation embryo development in cattle, we clarified dynamics of cathepsin B activity and its expression, localization of lysosomes and autophagosomes. And also, our data show that the excess cathepsin B activity is induced under conditions of the environmental stress of culture. On the other hand, developmental competence of embryos exposed to culture stress can be improved by inhibition of cathepsin B activity. In conclusion, these findings implicate that cathepsin B activity as one of the possible pathways that are responsible for developmental arrest caused by culture stress. Moreover, regulating cathepsin B would be contributed for the improvement of in vitro embryo production system.
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| Free Research Field |
動物生殖学
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