2015 Fiscal Year Final Research Report
Analysis of the novel dense granule protein in Toxoplasma gondii
Project/Area Number |
25850197
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Veterinary medical science
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Research Institution | Chubu University (2015) Obihiro University of Agriculture and Veterinary Medicine (2013-2014) |
Principal Investigator |
OKADA Tadashi 中部大学, 生命健康科学部, 研究員 (30623855)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Keywords | トキソプラズマ原虫 / dense granule protein / egress |
Outline of Final Research Achievements |
The intracellular protozoan parasite Toxoplasma gondii is capable of invading any nucleated cell and replicates within a parasitophorous vacuole (PV). This microenvironment is modified by secretory proteins from organelles named rhoptries and dense granules. In this study, we identified the novel dense granule protein in Toxoplasma gondii. To understand the novel dense granule protein function, we performed disruption of this gene by homologous recombination, and analyzed its phenotypes. The knock out strain showed similar growth rate as that of parental strain. The survival rate did not show significant difference between mice infected with knock out and parental strains. However, timing of egress was earlier than that of parental strain. These results suggested that this novel dense granule protein involved in regulating the parasite egress in T. gondii.
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Free Research Field |
分子生物学 生化学 寄生虫学
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