Contribution of chromatin remodeling complexes to the spatial positioning of DNA damage region and to the genome integrity

2018 Fiscal Year Final Research Report

• Project/Area Number: 25850242
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Tohoku University
• Principal Investigator: OMA Yukako 東北大学, 農学研究科, JSPS特別研究員(RPD) (20443997)
• Project Period (FY): 2014-02-01 – 2019-03-31
• Keywords: クロマチン構造変換複合体 / DNA損傷修復 / 姉妹染色分体間接着

Outline of Final Research Achievements

DNA double-strand breaks (DSBs) are the most serious type of DNA damage. It has been shown that DNA damage-induced sister chromatid cohesion facilitates homologous recombination repair (HR) at DSB proximal regions. We found that the damage induced-cohesion was impaired in yeast mutants defected in nuclear pore complex (NPC) or in the DSB relocation to NPC. We also observed that damage-induced cohesion is impaired in yeast cells in which the association of SUMO ligase with NPC is abolished. We also showed the possible involvement of cohesin in this machinery. These results, we propose a model that the DSB relocation to NPC promotes the cohesin SUMOylation by SUMO ligase associated with NPC. This machineryis supposed to contribute to the maintenance of genome stability through facilitating HR of DSBs.

Free Research Field

分子細胞生物学

Academic Significance and Societal Importance of the Research Achievements

本研究では、DNA 修復の実験材料として最も広く用いられている出芽酵母を用いて解析を行ったが、出芽酵母とヒトのクロマチン構造変換複合体の機能は保存されている。そのため、本研究でのDNA損傷領域の核内配置におけるクロマチン構造変換複合体の関与の解析は、核膜タンパク質の変異によって生じる早老症などの核膜病の原因や治療などの分野にも大きく貢献できる