2016 Fiscal Year Final Research Report
Analyses of recycling mechanism and function of vacuolar carbohydrate in yeast
| Project/Area Number |
25850246
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | nutrition sensing / carbohydrate metabolism |
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| Outline of Final Research Achievements |
To elucidate the recycling mechanism and function of vacuolar carbohydrate in yeast, I focused on regulatory mechanism of Ams1 enzyme respond to nutrition.
As a result, it was found that this enzyme was strongly upregulated respond to starvation at both transcription level and post-translationl level. It was also revealed that this enzyme was tightly suppressed by TORC1 in nutrient rich condition, but induced by a stress-induced transcritional factor and by protease dependent processing in the vacuoles. These results suggested that yeast may recycle degradation product of intracellular mannnose in the vacuole especially in starved condition.
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| Free Research Field |
細胞生物学
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