Development of photocontrollable peroxynitrite donor based on photoinduced electron transfer for biological application

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860009
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Chemical pharmacy
• Research Institution: Nagoya City University
• Principal Investigator: IEDA Naoya 名古屋市立大学, 薬学研究科(研究院), 助教 (00642026)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: パーオキシナイトライト / 一酸化窒素 / 活性窒素種 / 光ケージド化合物 / ケミカルバイオロジー

Outline of Final Research Achievements

We had developed a visible-light-controllable peroxynitrite generator based on photoinduced electron transfer. We discovered that one of synthesized compounds in the process was worked as a visible light controllable NO donor, named as NOBL-1. NOBL-1 could release NO in response to blue light in cells, confirmed by using fluorogenic NO probe, DAR-4M, and fluorescence microscope. Finally, we successfully controlled vasodilation, one of the main functions of NO, of rat aorta ex vivo with NOBL-1 and blue light. This compound is expected to be a chemical tool for NO research and a candidate as a chemotherapeutic reagent. In the next, we will develop a visible-light-controllable peroxynitrite generator with knowledge acquired in this research.

Free Research Field

ケミカルバイオロジー