Development of determination method for lysophospholipids by LC/MS/MS

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860021
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: SAIGUSA Daisuke 東北大学, 東北メディカル・メガバンク機構, 助教 (90545237)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: リゾリン脂質 / スフィンゴ脂質 / LC/MS/MS / レーザーマイクロダイセクション / ヒートスタビライザー / 微量組織切片

Outline of Final Research Achievements

In this study, we developed the analytical protocol for demonstrating the intracellular distribution and gradation and localization of lysophospholipids (LPL) using a LC/MS/MS. First, we developed an ultra-high sensitive method for LPL by LC/MS/MS coupled with a column switching. Second, we have evaluated the LPL decomposition in mouse liver by a heat stabilizer. Finally, we detected and determined LPL form the frozen sections of tissue (10 μm) of mice that was cut by laser micro dissection and determined LPL by LC/MS/MS.

Free Research Field

分析化学