Development of specific and sensitive method for analysis of protein S-nitrosylation using mass spectrometry

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860023
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology
• Principal Investigator: TSUMOTO HIROKI 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (00409385)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: S-ニトロシル化 / 質量分析 / プロテオーム

Outline of Final Research Achievements

Protein S-nitrosylation is an important post-translational modification in a wide range of biological processes. However, direct detection of S-nitrosylated peptide using mass spectrometry is difficult because of instability and low abundance. The aim of this study is to develop a specific and sensitive method for analysis of protein S-nitrosylation using mass spectrometry. It was demonstrated that triphenylphosphine ester derivative is useful for the selective and efficient reduction of S-NO bonds compared with ascorbate and the water-soluble phosphine TXPTS is useful for the detection of S-nitrosylated peptide using mass spectrometry.

Free Research Field

プロテオーム解析、質量分析