2014 Fiscal Year Final Research Report
Studies on the alkaloids of Apocynaceae
Project/Area Number |
25860084
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Natural medicines
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Research Institution | Hoshi University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Keywords | キョウチクトウ科 / アルカロイド / Apocynaceae |
Outline of Final Research Achievements |
Twelve new indole alkaloids including three novel skeletons were isolated from Voacanga grandifolia and Tabernaemontana corymbosa. The novel alkaloid, voacalgine A showed moderate cell growth inhibitory activities against HL-60 and HCT116 cells. 9-Methoxyellipticine (9-Mep) isolated from Tabernaemontana phymata specificaly inhibits C/EBPβ expression of murine macrophage cell line, RAW264.7 and murine pre-adipocyte cell line, MC3T3-G2/PA6. 9-Mep was found to inhibit C/EBPβ expression of both mRNA and protein level. Inhibition of RAW264.7 cells C/EBPβ expression led to attenuation of TNF-α, COX-2, and MCP-1 expression suggesting anti inflammatory activity. On the other hand, inhibition of MC3T3-G2/PA6 cells C/EBPβ expression interupted MC3T3-G2/PA6 differentation into adipocyte.
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Free Research Field |
天然物化学
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