• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2015 Fiscal Year Final Research Report

A structural study of VKORC1 for development of personalized medicine

Research Project

  • PDF
Project/Area Number 25860089
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Drug development chemistry
Research InstitutionTeikyo University (2015)
Showa University (2013-2014)

Principal Investigator

Kusakabe Yoshio  帝京大学, 薬学部, 講師 (30338537)

Project Period (FY) 2013-04-01 – 2016-03-31
Keywords個別化医療 / X線結晶構造解析 / ワルファリン / VKORC1
Outline of Final Research Achievements

To increase the expression of Homo Sapience VKORC1 (HsVKORC1) and Rattus Norvegicus VKORC1 (RnVKORC1), we performed the codon optimization and cloned the HsVKORC1 and RnVKORC1 gene into the expression vector. After co-transfection of insect cells with baculovirus DNA and the expression vector, the recombinant baculovirus was amplified to obtain a higher titer stock solution. Virus titer was detected virus infections and plaque formation.
While we performed homology modeling of HsVKORC1, RnVKORC1, and Bos taurus VKORC1(BtVKORC1), as their three-dimensional (3D) structures had not been reported. Then, we performed molecular docking of Warfarin bound to HsVKORC1 and RnVKORC1 in molecular docking studies. As a result, our study revealed the binding site and binding mode of Warfarin into HsVKORC1, RnVKORC1, and BtVKORC1 from our docking model.

Free Research Field

個別化医療

URL: 

Published: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi