Development of pulmonary siRNA liposomes with long-term retention in the lung based on the evaluations of real-time in vivo imaging and inhalation properties

2015 Fiscal Year Final Research Report

• Project/Area Number: 25860119
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Gifu Pharmaceutical University
• Principal Investigator: Tahara Kohei 岐阜薬科大学, 薬学部, 講師 (30454325)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 吸入製剤 / siRNA / リポソーム / 肺局所

Outline of Final Research Achievements

In this study, we investigated the feasibility of inhaled nucleic acid medicine (siRNA) formulation, notably siRNA loaded liposomes for pulmonary therapy using nebulization. The stabilities and inhaled characterization (aerodynamic particle sizes) of nebulized siRNA formulations were evaluated to evaluate the therapeutic potential of pulmonary siRNA using nebulizers. Furthermore, the behavior of surface-modified liposomes in the rat lung and whole body was monitored using a real-time in vivo imaging system. There results demonstrated that surface modification of liposomes using functional polymers achieved long-term residence time after pulmonary administration.

Free Research Field

薬物送達学