Differentiation of human iPS cells to pharmacokinetically functional enterocytes

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860120
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Nagoya City University
• Principal Investigator: IWAO Takahiro 名古屋市立大学, 薬学研究科(研究院), 講師 (50581740)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: iPS細胞 / 小腸 / 分化誘導 / 薬物動態

Outline of Final Research Achievements

We found several effective small-molecule compounds on inducing differentiation of human iPS cells to enterocytes and gain of pharmacokinetic function. The enterocyte-like cells differentiated by using these compounds expressed intestinal markers and exhibited drug-metabolizing enzyme activities, CYP3A4 inducibility, and active peptide transport. We demonstrated to culture the intestinal stem cell-like cells with differentiation capability for about 1 month and to cryopreserve the cells. These results would provided useful information for application of iPS cell-derived enterocytes to drug development studies.

Free Research Field

薬物動態学