2015 Fiscal Year Final Research Report
Functional analysis of novel polarity-related genes for hippocampal neuronal formation
| Project/Area Number |
25860141
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | University of Yamanashi (2015)
Osaka University (2013-2014) |
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Principal Investigator |
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| Research Collaborator |
HARADA Akihiro 大阪大学, 医学研究科, 教授 (40251441)
YOSHIMURA Shin-ichiro 大阪大学, 医学研究科, 助教 (60584521)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | ノックアウトマウス / 極性輸送 / 神経細胞 |
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| Outline of Final Research Achievements |
To know the functional relationship of novel polarity-related genes for hippocampal neuronal formation, I analyzed the genes which involved in the polarity formation using C. elegans. RNAi analysis. I performed the yeast two-hybrid system to identify the physical relationships of the candidate genes. However, I couldn't identify the significant result of their physical relationships. Therefore, I investigated the gene Rab1, whose knockdown showed significant abnormality in polarized epithelial cells of C.elegans. I analyzed the conditional knockout mice for Rab1a using Nestin-Cre, but can't see any significant abnormality in the behavior and the brain formation. This might come from the redundancy of Rab1b, which is a gene in Rab1 family. Therefore, I must analyze the Rab1a and 1b double knockout mice in the future research.
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| Free Research Field |
Neurogenesis
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