Hippo signal analysis during cardiac precursor cells migration in zebrafish

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860153
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: FUKUI Hajime 独立行政法人国立循環器病研究センター, 研究所, 研究員 (80551506)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: hippoシグナル / スフィンゴシン１リン酸 / ゼブラフィッシュ / 心臓発生

Outline of Final Research Achievements

To form the primary heart tube, bilateral cardiac precursor cells (CPCs) migrate toward the midline beneath the endoderm. Mutants of sphingosine 1-phosphate (S1P) signaling exhibit cardiac defect, whereas the cause of this defect in S1P signaling mutants remains unclear.
Here, we show that S1P siganling regulates CPCs migration through Yap1 dependent endoderm survival. Moreover, the Yap1-Tead transcriptional target Ctgfa also regulates CPCs migration. Finally, we found that both Yap1 and Ctgfa cell-autonomously regulate the endodermal sheet maintenance and is essential for the proper endoderm formation required for CPC migration.

Free Research Field

発生生物学