A role of neuronal calcium sensor-1 on the nuclear calcium regulation in cardiomyocytes: contribution to cardiac hypertrophy

2015 Fiscal Year Final Research Report

• Project/Area Number: 25860174
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: NAKAO Shu 国立研究開発法人国立循環器病研究センター, 研究所, 流動研究員 (30646956)
• Co-Investigator(Renkei-kenkyūsha): NAKAMURA Tomoe Y (西谷 友重) 国立研究開発法人国立循環器病研究センター, 研究所, 室長 (50393244) ; WAKABAYASHI Shigeo 国立研究開発法人国立循環器病研究センター, 研究所, 非常勤研究員 (70158583)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 細胞生物学 / 分子生物学 / カルシウム / 心肥大 / 心筋細胞 / カルシウムセンサー / 興奮収縮連関 / 受容体刺激

Outline of Final Research Achievements

To investigate the regulation of nuclear calcium in cardiomyocytes, we generated adenovirus vectors of cytoplasmic/nuclear-specific calcium probes GECOs, and induced them in mouse ventricular myocytes. Calcium imaging analysis revealed that a hypertrophy-associated hormone induced larger calcium transients in the nucleus than those in the cytoplasm. Using mice lacking a calcium sensor protein neuronal calcium censor-1 (NCS-1), which we previously reported as a crucial regulator of calcium signaling in cardiomyocytes, we demonstrated that NCS-1 contributes to the nuclear calcium regulation. Moreover, a protein/protein interaction assay exhibited that a NCS-1-dependent regulation of nuclear calcium is modulated through the interaction between NCS-1 and inositol-1,4,5 trisphosphate receptor, an intracellular calcium release channel, in cardiomyocytes.

Free Research Field

心臓生理学