2014 Fiscal Year Final Research Report
Structures that deliver the circadian rhythm from the suprachiasamtic nucleus to neighboring brain regions
| Project/Area Number |
25860181
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental physiology(including physical medicine and nutritional physiology)
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| Research Institution | Kinki University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 体内時計 / 視交叉上核 / 室傍核 / 組織培養 |
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| Outline of Final Research Achievements |
The suprachiasmatic nucleus (SCN) is the center of circadian clock. In order to delineate what biological structure transmits circadian rhythm to paraventricular nucleus (PVN) and subparaventricular zone (SPZ) from the SCN, we observed the coherence between the circadian rhythms in the SCN and PVN/SPZ by monitoring bioluminescence emitted from tissue slice from Per2::luc knock-in mice. In slices containing SCN and PVN/SPZ, the two regions showed antiphasic circadian rhythm, in contrast, slices containing PVN/SPZ but not SCN showed a circadian rhythm which was damped after a few days. However, when the slice showing damped oscillation was co-cultured with the SCN slice, PVN/SPZ restored a stable circadian rhythm antiphasic to that in the SCN. The findings suggest that the structure maintaining the coherence between the circadian oscillations in the SCN and PVN/SPZ was reconstructed.
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| Free Research Field |
時間生物学
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