2014 Fiscal Year Final Research Report
Identification of NOX1-expressing cells and pathophysiological roles of NOX1 in colon
| Project/Area Number |
25860191
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
MATSUMOTO Misaki 京都府立医科大学, 医学(系)研究科(研究院), 助教 (80533926)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 大腸 / 活性酸素種 / NADPH oxidase / 抗体 / マウス |
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| Outline of Final Research Achievements |
Specific monoclonal antibodies against mouse NOX1 (clone: 26B1, 31C1) were established through the screening using NOX1-knockout mice as a negative control. This novel antibody revealed that NOX1 was expressed in Ki-67-positive proliferating colonic epithelial cells but not in WGA-bound goblet cells, supporting a recent finding that NOX1 is involved in mucosal wound repair. In addition, anti-NOX1 antibody partially suppressed NOX1/NADPH oxidase activity, which suggests a possible usage as neutralizing antibodies.
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| Free Research Field |
薬理学
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