2014 Fiscal Year Final Research Report
Role of Rab GTPases in the intracellular logistics of insulin granule
| Project/Area Number |
25860208
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | insulin / Rab27a / β細胞 |
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| Outline of Final Research Achievements |
Regulated secretion is a main pathway for secretory cells to deliver bioactive molecules to the surface or outside of the cell. Although the molecular machinery for these processes have been characterized, precise molecular mechanisms are poorly understood. Previous studies have shown that the small GTPase Rab27 is required for late steps of this pathway, granule trafficking, docking, and fusion of insulin granule. We found that Rab27a forms a novel protein complex in pancreatic beta-cells. Although Rab27a localized on almost all granules, the complex specifically localized on immature granules. Furthermore, knockdown of Rab27a and that of other component of the complex decreased glucose-induced insulin secretion and conversion of proinsulin to insulin. These data suggest that the novel Rab27a complex is involved in the transition from granule maturation to secretion in the regulated secretory pathway.
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| Free Research Field |
分子生物学
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