Transdifferentiation of pancreatic exocrine cells to beta cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860213
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Osaka University
• Principal Investigator: MIYAZAKI Satsuki 大阪大学, 医学(系)研究科(研究院), 助教 (60452439)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 転写因子 / 腺房細胞 / インスリン

Outline of Final Research Achievements

I established a transgenic mouse line in which Pdx1 and Isl1 could be inducibly expressed in the acinar cells. My results showed that acinar-derived EGFP-positive insulin producing cells appear by induction of both Pdx1 and Isl1 in adult exocrine cells. Overexpression of the factors necessary for transdifferentiaion using lentivirul vectors in sorted EGFP-positive acinar cells induced acinar-to-beta cell transdifferentiation. Although further study is required, my result provides a promise for the in vitro transdifferentiation of exocrine cells to beta cells.

Free Research Field

再生医学