2014 Fiscal Year Final Research Report
The functional analysis of GATA2 in mature mast cells
Project/Area Number |
25860221
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
General medical chemistry
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Research Institution | Takasaki University of Health and Welfare |
Principal Investigator |
OHMORI Shin'ya 高崎健康福祉大学, 薬学部, 助手 (10509194)
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Project Period (FY) |
2013-04-01 – 2015-03-31
|
Keywords | 転写因子 / Gata2 / Cebpa / 細胞分化 / マスト細胞 / 脱分化 / 分化転換 |
Outline of Final Research Achievements |
To clarify roles GATA2 plays in the BMMCs, the DNA binding C-finger domain of GATA2 was genetically disrupted by Cre-LoxP system in BMMCs (G2ΔCF-BMMCs). We found that deletion of GATA2 CF results in a loss of mast cell identity of BMMCs, whereas the expression of Mac1- and/or Ly-6C/G-positive cells was significantly increased in G2ΔCF-BMMCs. Furthermore, GATA2 ablation led to a significant upregulation of C/EBPα, a key player of myeloid cell differentiation, and forced expression of C/EBPα in wild-type BMMCs phenocopied the GATA2ΔCF cells. Our findings suggest that the repression of Cebpa by GATA2 is essential for maintaining mast cell identity in BMMCs.
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Free Research Field |
分子生物学
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