2014 Fiscal Year Final Research Report
In vitro derivation of follicles from mouse ES cells and somatic cells
| Project/Area Number |
25860222
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | ES細胞 / iPS細胞 / 生殖細胞 / 卵胞形成 / 分化誘導 |
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| Outline of Final Research Achievements |
Folliculogenesis-competent ES cells higly expressed transcription factors which are important for folliculogenesis. Based on this finding, we transduced transcription factors essential for folliculogenesis into mouse iPS cells with germ cell-specific reporter gene. Among genes transduced, overexpression of Nobox and Figla induced derivation of germ cell-specific reporter+ cells. The induction was observed even when only Nobox was transduced; however, the efficiency was improved by a combination with Figla. Unexpectedly, overexpression of Nobox and Figla did not induce germ cell-specific reporter+ cells under cell differentiation culture conditions. Taken all together, we speculate that Nobox and Figla would bring pluripotent stem cells to 'metastable state' between pluripotency and germ cells, therefore the cells could turn to somatic cells under somatic cell differentiation cultures.
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| Free Research Field |
幹細胞生物学
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