Molecular understanding of gliomagenesis using neural differentiation sysytem

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860236
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: WATANABE Akira 京都大学, iPS細胞研究所, 助教 (60506765)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: シングルセル / 脳腫瘍

Outline of Final Research Achievements

The purpose of this study is to address the early event of gliomagenesis. I hypothesized that initiation of tumorigenesis is deviation from a normal neural differentiation. First, I established a methodology of single cell gene expression analysis, which enables to analyze dynamics of transcriptome during the differentiation. By combination with bioinformatical analysis, I successfully re-order the single cell samples in order to differentiation stage. Then, I applied this technique to neural differentiation, and demonstrated the dynamic alteration of transcription during neural differentiation. And also, I could identify cell populations that deviated from normal differentiation. The molecular mechanism that is responsible for branching may facilitate the molecular understanding of gliomagenesis.

Free Research Field

幹細胞生物学