Dynamic analyses of tumor-initiating cells in lymphoplasmacytic lymphoma (LPL)

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860268
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Osaka University
• Principal Investigator: WADA NAOKI 大阪大学, 医学(系)研究科(研究院), 助教 (80521731)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 腫瘍幹細胞 / 悪性リンパ腫

Outline of Final Research Achievements

MWCL-1 was established as LPL cell line. MWCL-1 cells were classified into three subpopulations using a flow cytometer with anti-CD20 and anti-CD138 antibodies: CD20－CD138－, CD20＋CD138－, and CD20＋CD138＋. When cultured, CD20－CD138－ cells yielded all three subpopulations. Compared to the other subpopulations, CD20－CD138－ cells possessed the efficient ROS expelling and in vitro colony formation activities, and were resistant to apoptosis. These results suggested that CD20－CD138－ cells might be a candidate for tumor-initiating cells in LPL. Low oxygen culture of MWCL-1 induced the production of CD20－CD138－ cells, and this result suggested that hypoxia was an advantageous microenvironment for CD20－CD138－ cells. CXCR7 mRNA/protein expression was the highest in CD20－CD138－ cells. When CXCL12, a ligand of CXCR7, was added, the proportion of CD20－CD138－ cells significantly increased. CXCL12-CXCR7 signaling might exert a great influence on CD20－CD138－ cells.

Free Research Field

悪性リンパ腫