2015 Fiscal Year Final Research Report
Comprehensive analysis of metalloproteinases in cancer-associated fibroblasts
| Project/Area Number |
25860276
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | がん / 線維芽細胞 / メタロプロテアーゼ |
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| Outline of Final Research Achievements |
Carcinoma-associated fibroblasts (CAFs), frequently observed in tumor stroma, are more capable of maintaining cancer stem cells and promoting tumor growth and angiogenesis through their interactions with carcinoma cells. In this study, we performed comprehensive analysis of metalloproteinases in tumor stroma and CAFs. We found that, in stroma associated with human cancer, several metalloproteinases were significantly upregulated, whereas there was a trend towards decreased expression of tissue inhibitors of metalloproteinases (TIMPs). By analyzing CAF microarray datasets, we identified several candidates for CAF-specific metalloproteinases. CAFs also secreted exosomes containing more metalloproteinases including ADAM10 compared to normal adjacent fibroblasts. These data suggest that dysregulation of metalloproteinases in CAFs may contribute to their tumor-propagating ability in patients.
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| Free Research Field |
実験病理学
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