2016 Fiscal Year Final Research Report
A novel dsRNA mycovirus reduces pathogenicity of Aspergillus fumigatus in a mouse infection model.
| Project/Area Number |
25860314
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Chiba University |
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Principal Investigator |
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| Research Collaborator |
URAYAMA Syun-ishi 東京農工大学
YAHARA Misa 千葉大学, 大学院生
SHISHIDO Erika 千葉大学, 大学院生
HIBIYA Yuko 国立がん研究センター
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | マイコウイルス |
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| Outline of Final Research Achievements |
In a screen of 44 clinical and environmental isolates of A. fumigatus for mycoviruses, four of the isolates were found to contain distinct groups of dsRNA segments. By using next-generation sequencer (illimina, Miseq), we have sequenced the dsRNA mycovitus genomes of these mycoviruses which are respectively members of the Chrysovirus genus, the Partitivirus genus, and 2 of novel mycoviruses. In this investigation, isogenic lines of virus-free and virus-infected A. fumigatus for each mycovirus infections have been compared with respect to phenotypic differences and effects on host fitness. Virulence assays in mouse model implicated that a novel mycovirus influence for normal pathogenesis. In addition, we also will report that mycovirus have influence on A. fumigatus characterization, such as, number of spores, mycelia morphology, adherence of A. fumigatus resting conidia to host pulmonary epithelial cells, and tolerance for macrophage phagocytosis.
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| Free Research Field |
医真菌学
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