To clarify why the stability of CDT-I prophage is different between Escherichia coli belonging to different O serogroups

2015 Fiscal Year Final Research Report

• Project/Area Number: 25860323
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: Osaka Prefecture University
• Principal Investigator: Atsushi Hinenoya 大阪府立大学, 生命環境科学研究科(系), 助教 (20548490)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: CDT / 大腸菌 / ファージ / spontaneous induction

Outline of Final Research Achievements

Cytolethal distending toxin (CDT-I) produced by Escherichia coli is encoded on lambdoid phage, and its phage induction is associated with the toxin production. We found that the induction level of CDT-I phage is different among the strains, and correlated with O serogroup of host E. coli including O127 and O142. The present study tried to identify the host factor which regulate CDT-I phage induction. Finally although we could not identify it during this research period, new findings could be obtained: (1) CDT-I phage induction is occurred in a RecA dependent pathway, and (2) intra-prophage interaction could be involved in the different CDT-I phage induction between CDT-I-producing E. coli O serogroups O127 and O142.

Free Research Field

細菌学