2014 Fiscal Year Final Research Report
The analysis of the mechanism of SARS coronavirus nsp1-mediated mRNA decay
| Project/Area Number |
25860338
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | University of Yamanashi |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | RNAウイルス / 病原性 / RNA分解 / コロナウイルス / 宿主因子 |
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| Outline of Final Research Achievements |
SARS coronavirus nsp1 protein causes a severe inhibition of host gene expression by inducing an endonucleolytic cleavage near the 5’UTR of host mRNA. However, the mechanism of nsp1-mediated mRNA cleavage is not known. In the current study, UPF1 protein, which plays a central role in nonsense-mediated RNA decay (NMD), was identified as the host factor interacting with nsp1 protein in cultured mammalian cells. The knockdowns of UPF1 and another NMD-related host protein reduced the efficiency of the nsp1-mediated mRNA degradation. These data suggested that nsp1 may recruit the NMD-related proteins on host mRNAs through the interaction with UPF1 protein.
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| Free Research Field |
ウイルス学
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