Analysis of hemorrhagic fever virus assembly and budding by visible technology

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860342
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Nagasaki University
• Principal Investigator: URATA Shuzo 長崎大学, 熱帯医学研究所, 助教 (20614449)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ウイルス

Outline of Final Research Achievements

1. Lassa virus Z has a mojor role on virus assembly and budding. We have shown that Z localized at late endosome(CD63 as a marker), and viral glycoprotein (GPC) facilitated this localization significantly. 2. Cellular factor, ALIX/AIP1, plays an important role on Z-mdeiated virus budding. 3. Z was tagged with Nano luc (Nluc) gene to establish high-through put screening system, and A549 stably expressing Z-Nluc cell line was cloned.

Free Research Field

ウイルス学