Real-time imaging of cell penetrating peptide (CPP) in the cytosol

2016 Fiscal Year Final Research Report

• Project/Area Number: 25860400
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied pharmacology
• Research Institution: Niigata University (2014-2016); Osaka Ohtani University (2013)
• Principal Investigator: Okuda Akiko (田所明子) 新潟大学, 医歯学系, 助教 (60454584)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 膜透過ペプチド / ドラッグデリバリー / エンドサイトーシス

Outline of Final Research Achievements

Cell-penetrating peptides (CPPs) can enter cells with cargo. However, endocytosis plays a major role in their uptake and keep most of CPP-cargo trapped in endosomes. In this study, I designed peptides which could green fluorescent protein (EGFP) and immunoglobulin (IgG) into cytosol of HEK293. Furthermore, it was indicated that cellular uptake of CPP-EGFP and cytosolic delivery occur via endocytosis.

Free Research Field

分子生物学