Urinary exosomes as in-situ biomarker of renal epithelial cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860413
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Bunkyo Gakuin University
• Principal Investigator: AKI Nakayama 文京学院大学, 保健医療学部, 助手 (00599425)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: エキソソーム / 尿タンパク質 / バイオマーカー / 腎機能

Outline of Final Research Achievements

We identified urinary exosomal proteins by two dimensional electrophoresis-based proteome analyses which include membranous, extracellular region, and cytoplasm of renal epithelial cells. The numbers, diameters, and concentration of protein of exosomal fraction were consistent between healthy subjects. Furthermore, total protein concentration of urinary exosome fraction was consistent per unit time. This research provides the basic information about protein profile and amount of exosomal production in renal epithelial cell of healthy subjects.

Free Research Field

病態医科学