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2015 Fiscal Year Annual Research Report

非アルコール性脂肪性肝炎の病態形成におけるサイトグロビンの関与

Research Project

Project/Area Number 25860554
Research InstitutionOsaka City University

Principal Investigator

LE THUY  大阪市立大学, 大学院医学研究科, 特任助教 (10572175)

Project Period (FY) 2013-04-01 – 2016-03-31
Keywords遺伝子 / サイトグロビン
Outline of Annual Research Achievements

We claify the role of cytoglobin in the development of the liver fibrosis and cancer in humans and in a mouse model of non-alcoholic steatohepatitis.CYGB was expressed in HSCs of intact human livers,and its expression was reduced in NASH and HCC patients.In Cygb-/-mice,71.3% of those aged 1 to 2 years spontaneously exhibited abnormalities and cancer development in multiple organs,including the liver,lung,lymph nodes and heart,compared with 5.8% in WT mice.In the NASH model,CDAA treatment for 8weeks induced rubust inflammation,fibrosis,and prominent expression of γ-H2AX,a marker of oxidative stress-related DNA double-stranded breaks,in Cygb-/- livers. Interestingly,100% of Cygb-/- mice fed CDAA developed liver cancer,while no tumors were found in WT controls.An imbalance between oxidative stress and antioxidant defense in Cygb-/- mice fed CDAA was demonstrated by the altered expression of 31 ROS metabolism genes,including myeloperoxidase and glutathione peroxidase2,both of which are involved in H2O2 production.In addition,Cygb-/- mice exhibited induction of nitric oxide synthase,high levels of nitrotyrosine formation,and an increased number of hypoxic hepatocytes,as determined by pimonidzole staining.NAC treatment rescued oxidative stress conditions in HSCs in primary culture and attenuated liver tumor formation in CDAA-treated Cygb-/- mice.Conclution:These results indicate that CYGB may control ROS production in chronically inflamed liver and provide important insights into the role of CYGB expressed in HSCs in liver hemeostasis,fibrosis and cancer development.

  • Research Products

    (10 results)

All 2016 2015

All Journal Article (4 results) Presentation (5 results) (of which Int'l Joint Research: 3 results) Book (1 results)

  • [Journal Article] Discovery of cytoglobin and its roles in physiology and pathology of hepatic stellate cells.2016

    • Author(s)
      Yoshizato K,Thuy le TT, Shiota G,Kawada N.
    • Journal Title

      Proc Jpn Acad Ser B Phys Biol Sci.

      Volume: 92 Pages: 77-97

    • DOI

      10.2183/pjab.92.77

  • [Journal Article] Pathophysiological role of cytoglobin,the fourth globin in mammals,in liver diseases.2016

    • Author(s)
      Thuy le TT,Hai NT,Hai H,Kawada N.
    • Journal Title

      Histol Histopathol.

      Volume: 31 Pages: 257-267

  • [Journal Article] Cytoglobin Deficiency Promotes Liver Cancer Development from Hepatosteatosis through Activation of the Oxidative Stress Pathway.2015

    • Author(s)
      Thuy le TT,Matsumoto Y,Thuy Tuong TV, Hai H,Suoh M,Urahara Y,Motoyama H,Fujii H,Tamori A,Kubo S,Takemura S,Morita T,Yoshizato K, and Kawada N.
    • Journal Title

      Am J Pathol.

      Volume: 185 Pages: 1045-1060

  • [Journal Article] Cytoglobin expressed in hepatoc stellate cells affects an acute hepatocyte damage via alteration of CYP2E1-mediated xenobiotic metabolism.2015

    • Author(s)
      Teranishi Y,Matsubara T,Krausz K.W.,Thuy le TT,Gonzalez F. J.,Yoshizato K,Ikeda K,Kawada N.
    • Journal Title

      Lab Lab Invest.

      Volume: 95 Pages: 515-524

  • [Presentation] Protective Role of Cytoglobin in Liver Inflammation and Fibrogenesis in Mouse Steatohepatitis Model.2016

    • Author(s)
      Thuy le TT
    • Organizer
      APASL2016
    • Place of Presentation
      グランドプリンスホテル新高輪(東京都・港区)
    • Year and Date
      2016-02-20 – 2016-02-24
    • Int'l Joint Research
  • [Presentation] Loss of Cytoglobin Exacerbates Liver Fibrosis and Cancer Development in Steatohepatitis through the activation of Oxidative Stress Pathaway.2015

    • Author(s)
      THuy le TT
    • Organizer
      AASLD
    • Place of Presentation
      サンフランシスコ(アメリカ合衆国)
    • Year and Date
      2015-11-13 – 2015-11-17
    • Int'l Joint Research
  • [Presentation] Loss of Cygb accelerates liver fibrosis and cancer development despite of its etiology.2015

    • Author(s)
      Thuy le TT
    • Organizer
      ISHSR
    • Place of Presentation
      アシロマ(アメリカ合衆国)
    • Year and Date
      2015-11-11 – 2015-11-13
    • Int'l Joint Research
  • [Presentation] Absence of Cytoglobin, a Specific Marker of Hepatic Stellate Cells,Promotes Multiple Organ Abnormality in Aged Mice via Increased Nitric Oxide.2015

    • Author(s)
      Thuy le TT
    • Organizer
      第29回肝類洞壁細胞研究会学術集会
    • Place of Presentation
      秋田市にぎわい交流館AU(秋田県・秋田市)
    • Year and Date
      2015-10-31 – 2015-11-01
  • [Presentation] Cytoglobin Deficiency Promotes Liver Cancer Development from Hepatosteatosis through Activation of the Oxidative Stress Pathaway.2015

    • Author(s)
      Thuy le TT
    • Organizer
      第51回日本肝臓学会総会
    • Place of Presentation
      ホテル日航熊本(熊本県・熊本市)
    • Year and Date
      2015-05-21 – 2015-05-22
  • [Book] Stress in Applied Basic Reserch and Clinical Practice. CHAPTER 18:Antioxidant Approach to the Therapy of Chronic Liver Diseases.2015

    • Author(s)
      Le Thi Thanh Thuy,Hoang Hai,Norifumi Kawada
    • Total Pages
      413(389-413)
    • Publisher
      Springer International Publishing Switzerland

URL: 

Published: 2017-01-06  

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