High-throughput screening for drug discovery targeting gastrointestinal cancer stem cell.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860560
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Keio University
• Principal Investigator: TAKANO Ai 慶應義塾大学, 医学部, 研究員 (50647584)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 消化器がん / HTS

Outline of Final Research Achievements

We obtained cancer tissues from patients with gastrointestinal cancer. Using previously published protocol for colorectal cancer organoids (Sato T. Nature 2009), we have established various kinds of tumor organoids including gastric cancer, pancreatic cancer, bile duct cancer and gallbladder cancer. The established gastrointestinal cancer organoids can be cultured in a 384-well microplate format for High-throughput drug screen system. Image analysis was performed with 364 drugs on tumor organoids using High Contents Analyzer, which is the automated acquisition and analysis of images by object recognition and feature quantitation algorithms. Exploiting gene editing system, we knocked-in LGR5 reporter in colorectal cancer organoids for High- Throughput Screen (HTS). Our results suggest that HTS for tumor organids will be applicable for genetic mutation based drug discovery and personalized medicine.

Free Research Field

下部消化管学